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First ZEN paper out at Ecology Letters!

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Pamela Reynolds in the field at the ZEN site in Virginia

Pamela Reynolds in the field at the ZEN site in Virginia

by Pamela Reynolds (ZEN coordinator and Postdoc)

We are very excited that our first ZEN cross-site compartative experimental paper is in press in Ecology Letters!

The paper can be accessed here: http://onlinelibrary.wiley.com/doi/10.1111/ele.12448/abstract.

Follow us on Twitter @ZENscientists and Facebook at the Zostera Experimental Network for more updates. Also, I’ll be live tweeting about the new paper throughout the day today @PLNReynolds. Direct message me if you have any questions!

ZEN Meeting in Quebec City, Canada

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by Pamela Reynolds (ZEN coordinator & postdoc)

ZEN partners at the Benthic Ecology Meeting in Quebec City, March 2015

ZEN partners at the Benthic Ecology Meeting in Quebec City, March 2015

Earlier this month over 30 ZEN participants – faculty, students and staff from over half of our partner sites – met at the annual Benthic Ecology Meeting.

The Benthic Ecology Meeting 2015 at the Chateau Frontenac, Quebec City

The Benthic Ecology Meeting 2015 at the Chateau Frontenac, Quebec City

This year the meeting was held in the stunning Chateau Frontenac overlooking the St. Lawrence River in Quebec City, Quebec, Canada. While it was cold and snowy outside, the engaging science made it warm and lively inside. Our ZEN partners presented on their local research – on topics from seagrass habitat fragmentation and mesograzer complementarity, to mutualisms, disease dynamics, biodiversity, and restoration.

Jay Stachowicz talks about the role of eelgrass genotypic diversity at BEM

Jay Stachowicz talks about the role of eelgrass genotypic diversity at BEM

While the other meeting participants spent the open session on Friday afternoon to get out of the hotel and explore Quebec’s snowsports, our ZEN partners showed their true dedication by staying to discuss more science and plan for our upcoming field season. We even had 7 ZEN partners – from Vancouver to Sweden – who couldn’t be there in person Skype into our ZEN meeting! These virtual participants engaged with eachother and the in-person meeting attendees during breakout sessions. The feedback from all of the partners was great, and it was a nice opportunity for us to spend some real face time together.

Saint Lawrence River

Saint Lawrence River

Boat ice sculpture in downtown Quebec City

Boat ice sculpture in downtown Quebec City

And on the last day of the meeting we did get out a little bit to see the ice floating down the river and explore Quebec City. My favorite was the intricate ice sculptures throughout the city. I even tried the maple syrup on snow, a delightful taffy-like concoction, and did some ZEN “team bonding” with a sledding adventure.

The 2015 BEM had perhaps the best logo to date!

The 2015 BEM had perhaps the best logo to date!

Special thanks to Ladd Johnson and ZEN partner Mathieu Cusson for organizing a wonderful BEM and for facilitating our ZEN meeting.

A little closer to home – ZEN in Long Island Sound

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College of William and Mary undergraduate Ally Farnan

College of William and Mary undergraduate Ally Farnan

by Ally Farnan (College of William and Mary undergraduate, ZENtern)

Even though Long Island is not a conventionally exotic location for a girl from New Jersey to spend the summer, it is starting out to be quite an adventure.

I arrived on site less than two weeks ago and I already feel like a part of the family here in the Peterson lab. I have spent the past week prepping for the ZEN experiments in the lab. Though a crucial step in the process of running experiments and conducting research, spending a week making aluminum packets, labeling, and weighing tins is not exactly thrilling. It is, however, a necessary part of the process, and is giving me some great practice for the eventual post-processing, sorting, and identification of field samples yet to come. I’m still figuring out where everything in the lab is located, but am getting some excellent help from the other undergraduate volunteers working the lab.

Ally is spending this summer working with ZEN partners in Long Island

Ally is spending this summer working with ZEN partners in Long Island

Luckily, I get to start running the actual experiments on Monday, which will also be the first time that I get to see the seagrass bed that we will be working in. Since I have been so busy in the lab, I haven’t had the opportunity to get into my wetsuit and see the site. The area that we are located in is beautiful, so I expect that the study site will also be wonderful!

I’m living a short drive from the lab with two graduate students, which gives me an interesting perspective on how different science research is from the typical 9-5 business job. My housemates, Rebecca and Sam, are excellent hosts and it’s so much fun to hear about the different kinds of research that they do. It should be really exciting to delve further into the experimental stages of ZEN so I can gain some hands-on experience myself.

The lab here is nearby, just off the water. The weather has been great most days, so I’ve been able to explore the area by jogging around town and by the beach. I hope to see some more of the sights around where I am staying. All of my new friends in the lab have given me some great ideas for how to spend my free time during the upcoming weekends - I can’t wait to discover what makes Long Island so special.

Preparing for ZEN fieldwork in Long Island

Preparing for ZEN fieldwork in Long Island

It is surprisingly chilly here, so I haven’t been brave enough to go swimming just yet since my toes freeze every time I stick them in the water. But, the sun is constantly shining, making for some beautiful drives around town with my windows down. I can’t wait to continue my adventures here by getting some fieldwork experience. I’ve already packed up my wetsuit so I don’t freeze in the cold water!

I’m so lucky to have a bunch of help in the lab, not only to settle me into a new space and help me to learn more about seagrass ecosystems, but also to advise me on some cold weather gear for our fieldwork in the next few weeks! I’m sure my future blog posts will be filled with some exciting fieldwork stories and hopefully I’ll still have a little free time to explore the area and tell you more about those experiences as well!

A close look at a seagrass creature – caprellids

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Students in the UC Davis ZEN class sort mesograzers - small invertebrates that live in seagrass beds

Students in the UC Davis ZEN class sort specimens of mesograzers – small invertebrates that live in seagrass beds

by Elena Huynh (undergraduate in the UC Davis ZEN class)

My first look under the dissecting microscope at a caprellid amphipod was alarming, to say the least. Enormous Claw-like mouthparts and red eyes stared straight back at me and I jumped back in my seat. Lucky for me, no one was there to observe my visceral reaction to this strange looking animal. After taking a moment, I knew that I would need to get comfortable looking at these odd creatures since we would be performing lots of species identifications during the course of the ZEN class at UCD, so I gave it another look under the scope. Despite the permanent menacing stare, this caprellid was not so scary, albeit a bit bizarre. I noticed a deadly looking spike behind its head and paused before learning that this protuberance is a defining characteristic of this caprellid species—Caprella californica!

Caprellids on eelgrass

Caprellid amphipods clinging onto eelgrass

After observing this caprellid’s scythe-like claws, I wondered what they were used for. As it turns out, caprellids are pretty clingy. They use their claws (gnathopods) and their legs (pereopods) to hang onto seagrass leaves and algae as they either graze the epiphytes that grow on top of the leaves or filter particles out of the water. Some caprellids can actually help seagrasses grow by cleaning the seagrass leaves and increasing their light exposure, increasing their growth. I guess caprellids would make good housekeepers, since they’re constantly cleaning off dirty surfaces.

Out in the field, I had the opportunity to watch caprellids swim. I wondered how these stick figures managed to get around. Through close observation, I noticed that they do a lot of crawling – which makes sense because they don’t appear to have pronounced flippers or large paddles. When they are not clinging to a surface or crawling they get around by moving like a wave. They curl up their bodies then push their feet and head back at the same time, over and over. It is pretty surprising to see what they manage to do with a body plan I’d consider adverse to swimming.

After doing a little research, I found out that some caprellids have hairs on their antennae – called swimming setae – that help them get around (Caine 1979). Although some can swim, they don’t swim very well nor do they swim for very long. If they’re living in beds of seagrass, all they would really need to be able to do is to hop to get from leaf to leaf. Learning more about what caprellids do for a living has helped me appreciate them, and they just might now be my favorite inhabitants of seagrass beds!

For more information, check out: Caine E.A. Functions of Swimming Setae within Caprellid Amphipods (Crustacea). 1979. Biological Bulletin 156:169-178.

“Bok” from Croatia!

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College of William and Mary undergraduate Austin

College of William and Mary undergraduate Austin Ruhf

by Austin Ruhf (College of William and Mary undergraduate, ZENtern)

Bok, or hello, from Croatia! I’m one of the ZENterns from the College of William and Mary and am spending my summer with classmate Dave Godschalk assisting with the ZEN research in beautiful Zadar, Croatia. I have only been at my host site for a week, but so far Croatia has been a welcoming and easy going paradise. Despite a comical amount of initial bureaucracy (it seemed like we needed the equivalent of a social security registration to gain internet access), the locals have been more than accommodating towards my ignorant American self. My host supervisor at the University of Zadar, Dr. Claudia Kruschel, has been fantastic to work with. She seems to never grow tired and goes out of her way to make sure that all of our work is a group effort. She even lent me her daughter’s guitar so I wouldn’t get musical withdrawal during my stay abroad!

The ZEN team in Croatia prepares for fieldwork

The ZEN team in Croatia prepares for fieldwork

Dave and Austin prepare experimental materials in the laboratory

Dave and Austin prepare experimental materials in the laboratory

The only downside is that the ZEN field sites are subtidal and are so deep that they have to be surveyed by professional divers. This means that the bulk of my and Dave’s work is done in the lab. The facilities are setup differently from the lab back at VIMS, but the work itself is similar. After a strenuous first week, we have settled into a manageable schedule.

Outside of the lab, fellow intern Dave and I have begun to explore Zadar in our spare time. Dave is already hooked on espresso and sparkling water, and I’m trying to discover more about the culture of the young people here. This led me to watch a heavy metal music show in an old puppet theater, which was pretty strange but somehow felt completely natural. We next hope to visit a few of the world-famous beaches here in Croatia, and learn how to best avoid the crowds of German tourists. Who knew that this was such a popular spot for science, and for a holiday?

A buoyant day in the field

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San Diego State University undergraduate Whitney

San Diego State University undergraduate Whitney Dailey

by Whitney Dailey (San Diego State University undergraduate, ZENtern)

One of my favorite parts of the ZEN seagrass ecosystem ecology course at San Diego State University this past spring semester was working in the field and learning about seagrass ecology through a hands on approach.

One field day that I will never forget was when we were collecting our predation intensity experiment. The experiment consists of deploying several “tethers”, which are made by gluing grazers (in this case small grass shrimp or snails) to fishing line placed out in the seagrass bed. We then come back later to record whether the animals have been eaten. The purpose of the experiment is to understand the level of predation in the environment and the relatively susceptibility of different types of grazers.

Whitney and classmates learn the importance of being prepared for the unexpected - and in bringing weight belts out into the field

Whitney and classmates at SDSU learn the importance of being prepared for the unexpected – and in bringing weight belts out into the field

However, one of the main lessons that we learned during our time in the field with the ZEN class is that every experiment has its own challenges and the trick is to problem solve and find a way around it. On this particular field day we met the challenge of trying to schedule fieldwork around both our class schedule and the tide. For us this meant struggling to find the tethers in around 3 to 4 feet (1-1.5 meters) of water. While this may not sound challenging, we found it to be very difficult. We would try to dive down, but the extra buoyancy from our wetsuits at that shallow depth made us pop up to the surface right away. So, we just floated on the surface and would reach down and try to find them and pull them out. The trick was not giving up, and remembering to bring a weight belt in the future.

After many failed attempts of trying to retrieve the tethers, we were finally able to hold onto the pieces of rebar we’d installed to mark our experimental plots, and feel around until we found the tether and pulled it out. Every time we found a tether we would get so excited – until we moved on to retrieve the next one. But, with a lot of commitment and perseverance we completed our field day at our ZEN site in San Diego Bay and learned new methods for problem solving during fieldwork. We also learned that in marine ecology (and likely the other sciences) the truly exciting part comes from overcoming challenges, even the ones you didn’t anticipate!

Whitney graduated summa cum laude this May from San Diego State University, receiving her Bachelors of Science in Biology and Environmental Science. She has been working in seagrass ecology as part of Dr. Kevin Hovel’s lab for the past four years including on the initial ZEN projects in 2011-2012. Hailing from Washington, Whitney enjoys playing basketball and taking photographs in her spare time. This fall she plans to being a job as a research technician before applying to graduate school. Whitney will be traveling to work with Dr. Per-Olav Moksnes and his research group in Sweden as par of her ZENternship this summer.

A closer look at an eelgrass inhabitant – the Dungeness crab

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UCD undergraduate Jason Toy

UCD undergraduate Jason Toy

by Jason Toy (Undergraduate student in the UCD ZEN class)

Several weeks ago, I was out at Bodega Bay with my classmates from the UCD ZEN course collecting field data for our research projects.  As I stood out there in the water in my borrowed waders, I observed quite a few different species of invertebrates as they made their way through the bed of eelgrass.  Most were quite familiar to me at that point, but there were a few species, including the Dungeness crab, Cancer magister, that I had not thought of as typical eelgrass inhabitants.  I decided to do a little research on the life history of these crustaceans in order to better understand the importance of estuarine seagrass beds to this – and potentially other – important crustacean species.

Life cycle of the Dungeness crab (Tasto et al. 1983)

 

I was surprised to learn that these crabs move around quite a bit during their life histories.  Adults mate in nearshore coastal locations in the Pacific Northwest throughout the spring (Pauley et al., 1989), a process in which the male embraces the female for up to 7 days before she molts, after which the actual transfer of sperm occurs. She stores this sperm for about a month until she extrudes her eggs and, in the process of doing so, they are fertilized (Tasto et al., 1893).  But her care doesn’t end there.  She protects her eggs by storing them on her abdomen for several months until winter (typically December – January). Then one day, these 1-2 million eggs hatch and the baby crabs leave their mother and enter a 105-125 day planktonic larval period. During this time the young Dungeness crab goes through many developmental changes, moving through 5 zoeal and 1 megalopal stages.  After reaching the megalopa stage, the young crabs settle out onto the bottoms of bays and estuaries, where they molt into their first juvenile crab stage and begin to actually resemble real crabs.

During field sampling, Jason and classmates found a few juvenile Dungeness crabs hiding amid the eelgrass in Bodega Bay, California

During field sampling, Jason and classmates found a few juvenile Dungeness crabs hiding amid the eelgrass in Bodega Bay, California

This is where seagrass comes in!  Coastal estuaries (like Bodega Harbor) play a critical role for juvenile Dungeness crabs as a nursery.  Large numbers of juvenile crabs benefit from the protection and substrate provided by beds of eelgrass (Zostera marina). They cling to and hide within the grass, consuming other small organisms within the habitat (amphipods, isopods, polychaetes, essentially whatever they can catch!) (Pauley et al., 1989).  After several molts, subadults and adult Dungeness crabs begin to leave the eelgrass beds and move offshore, but a few do remain in inland coastal waters, hiding among the eelgrass blades.

Dr. Stachowicz and Jason pause for a quick photo during the class field trip

Dr. Stachowicz and Jason pause for a quick photo during the class field trip

The role of seagrass beds in the life history of Dungeness crabs is an important interaction to talk about because it is an example of an economic benefit (known as an ecosystem service) provided by seagrass beds.  Seagrasses are critical species in coastal environments around the globe, as they form habitat structure and promote a large diversity of organisms.  However, like the coral reefs, they are currently in decline due to the effects of human activities.  The connection of an economically important species such as Cancer magister to these seagrass ecosystems, however, can help bring attention to this issue, and provide an incentive for the preservation and restoration of this habitat. Help spread the word!

For more information, check out these references:

Pauley, G. B., Armstrong, D. A., Citter, R. V., & Thomas, G. L. (1989). Species Profiles: Life Histories and  Environmental Requirements of Coastal Fishes and Invertebrates (Pacific Southwest): Dungeness Crab. U.S. Fish and Wildlife Services Biological Report 82(11.121): 7-8.

Tasto, R. N., & Wild, P. W. (1983) Life History, Environment, and Mariculture Studies of the Dungeness   Crab, Cancer Magister, With Emphasis on The Central California Fishery Resource. California  Department of Fish and Game Fish Bulletin 172, 319-320. Retrieved June 11, 2014, from http://content.cdlib.org/view?docId=kt1k4001gs&&doc.view=entire_text

Jason is an aspiring marine ecologist with a passion for all things science. He is a rising senior at UC Davis studying evolution and ecology. His dream job is to be a research SCUBA diver. This summer he will be working in Dr. Jay Stachowicz’s marine ecology lab at UC Davis’ Bodega Marine Laboratory.

Loving science for the challenge

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San Diego State University undergraduate Chris Bayne

San Diego State University undergraduate Chris Bayne

by Christopher Bayne (San Diego State University undergraduate, ZENtern)

Field days have to be one of my favorite parts of science for two main reasons. The first reason is fairly obvious – we get to go out to the field site, which means getting some exercise, being in the sun, and working in the natural environment. You can’t beat that! The second reason is less intuitive. I really enjoy the challenges of fieldwork and problem solving. No matter how well protocols are planned and how many times you go over them, it seems that the smallest thing can turn into the biggest pain. Even though these problems can be time consuming and counterproductive, they keep you on your toes.

Along those lines, the ZEN predation intensity experiment comes to mind. The first time we deployed the experiment, which tested different materials as potential standardized prey to be used in experiments conducted by all of the ZEN partners, we used braided line threaded through different bait types. We separated each bait into separate bags for “quick and easy” deployment in the field. However, when we reached our field site we soon discovered that, when braided line goes into a bag, the mixture of moving around, getting wet, and Murphy’s law results in a knot so bad that you can barely distinguish one line from another. It just so happens that this occurred to six of our eight different bait types. What do we do?

The ZEN SDSU class conducting field experiments in the eelgrass in San Diego Bay

The ZEN SDSU class conducting field experiments in the eelgrass in San Diego Bay

We approached the problem like you do with so many other unexpected field issues. You accept it and just jump in headfirst.  You approach the knot and honestly, there is no apparent advantage of coming at it at any certain way. You just begin trying to separate each line one at a time. You try different things: weaving the bait end through, loosening the most knotted sections, pulling the line through slowly. You swear you’ve undone hundreds of knots yet the ball of tangled string never looks as if any progress was made. Finally, you notice one looks as if it is almost loose. You put all your effort into that and get it out with this silent victory to yourself. Eventually they are all loose and you re-wrap them in a way that they will not get tangled up again. You think about what you could or should have done to avoid this mess. You finish up what needs to be done, knowing you are ready for whatever comes next.

In the end, the problem of the knotted line may be small but it is symbolic of the problems researchers face all the time. Little complications creep out of nowhere, completely unexpected. You try a million different solutions and begin to loose faith when nothing seems to work. If you have enough patience, the knot will eventually loosen and you will be victorious, swearing that you will never make that mistake again. This is why I have chosen to do science. It is challenging in so many ways. Learning the correct terminology, setting up experiments, finding solutions to unexpected problems – these are just some of the reasons why science is challenging and yet very rewarding.

Working on a project as large as ZEN adds an additional level of complexity in both coordinating large teams of people distributed all over the globe. This summer I will be travelling to Japan to work with the ZEN partners in both Akkeshi and Hiroshima. It is an amazing opportunity that not many undergraduates get to be a part of. I am looking forward to all of the challenges I will be facing this summer. I’m sure there will be more than a few but, if I’ve learned my lesson, tangled fishing lines will not be one of them!

Chris is a rising senior at San Diego State University pursuing his bachelor’s in biology and Japanese. Before starting the ZEN program Chris worked with Dr. Violet Compton Renick on her dissertation research examining the interactive effects of parasites and pesticides on killifish behavior. During the SDSU ZEN course Chris led a feeding experiment where he measured the grazing rates of several different types of San Diego mesograzer. This summer Chris will be traveling as part of his ZENternship to Japan to assist Drs. Massa Nakaoka and Massakazu Hori. 

Thowback Thursday – Setting the Stage

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Experimental gear shipped to ZEN partners in 2011

Experimental gear shipped to ZEN partners in 2011

Working with a large, global network of collaborators poses unique opportunities — as well as challenges. Before ZEN’s parallel experiments could start at the 15 widely scattered partner sites in 2011, the VIMS team had to purchase, fabricate, assemble, package, and ship the experimental materials to the sites located throughout the northern hemisphere. That summer we shipped out up to 6 crates and over 500 lbs of gear per site (that’s almost 3.8 tons total!). There was a lot to be done before we could pull on the dive booties and plunge into the water at our own site! For summer of 2014 the focus is on rigorous, controlled surveys and smaller scale experiments, which has the added benefit of less international shipping!

Tsawwassen – home to big isopods, eelgrass and crabs

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College of William & Mary undergraduate Danielle Hall

College of William & Mary undergraduate Danielle Hall

by Danielle Hall (College of William & Mary undergraduate student, ZENtern)

After three days of intense lab preparation and field work, the first ZEN2 site has been completed!

I arrived in British Columbia late on May 15th and the next day we got straight to work. After prepping materials in the lab we hopped in the lab truck and headed to our field site in a town outside of Vancouver called Tsawwassen. This particular site is in an intertidal zone and we chose to work on the lowest tide of the year since, during any other time, the site would be submerged and difficult to access.

To get to the site we climbed down over two hundred steps. Next, in our fashionable rubber boots, we hiked our way through the mud to get to our site (a treacherous task since the mud had a habit of clinging to our boots). On more than one occasion help was required to extricate a sunken foot.

I was amazed at the size of the Zostera shoots. They were nearly twice the size of the eelgrass in my home field site in Virginia! Not only were the shoots large, but the isopods were comparatively gigantic as well.

Marking experimental plots in the eelgrass bed in Vancouver, British Columbia

Marking experimental plots in the eelgrass bed in Vancouver, British Columbia

I was joining on day two of the field work and so the 20 experimental plots were already marked with orange flags. On this day, our job was to deploy the predation assay. The purpose of this assay is to understand the relative predation rates across the different ZEN site. The experimental units, or ‘PTUs,’ consist of a piece of bait tethered to an acrylic rod. The baits ranged from local animals, like the giant isopods, to a standard control, a piece of cut dried squid. A PVC quadrat helped visualize where to place our PTUs.

Inside the eelgrass in Vancouver, BC

A hermit crab crawls along the eelgrass bed in Vancouver, BC

Having spent several summers helping deploy previous ZEN projects in the Chesapeake Bay, I was very curious to discover the differences in eelgrass beds between the two coasts. Here, anemones abounded right on the eelgrass shoots. I was amazed that they were able to make a living on plant tissue since in all my past experiences I had only ever seen anemones attached to a hard substrate (There are anemones living in eelgrass beds in the Chesapeake Bay as well, but they are generally small and scarce.)

Nudibranch

Nudibranch

Our lab group got particularly excited about a pair of nudibranchs who then became the stars of a Hollywood-esque photoshoot. Meanwhile a bald eagle joined us for a bit, clearly finding a tasty meal within the seagrass.

The BC team poses with a Dungeness crab found in the eelgrass bed

The BC team poses with a Dungeness crab found in the eelgrass bed

Our second day in the field was filled with surprises. After recording the results of the predation assay (the bait was either gone – eaten, presumably – or present), we continued with collecting samples. After combing through the plots at least two times, Celine – another ZEN participant – uncovered quite a surprise: a huge Dungeness crab hiding in the mud! And he was not too happy that we were intruding in his home. One of the other women, Jemma, mustered up the guts to pick up the feisty crab, and we all gathered around to take a picture with our new friend.

The ZEN team in BC hard at work

The ZEN team in BC hard at work

Day two was a long afternoon of working in our plots. So long in fact that the tide decided to turn despite the fact that we still had half our plots to process. A race ensued. We were optimistic. The tide was persistent. We lost. I was in the process of taking sediment core samples when I noticed the tide line getting ever so close, though I was determined to finish. When I peeked over my shoulder, I decided I could finish three more plots. I didn’t even finish the one I was on.  Soon water was rushing into our site.

Danielle (left) and the BC team celebrate a successful ZEN sampling

Danielle (left) and the BC team celebrate a successful ZEN sampling

I abandoned my own task to help others finish collecting algae. We developed an effective method of the two hand scoop, where we quickly ran both hands through the plot to work like a sieve. Soon the water engulfed our boots which was followed by multiple exclamations, but together we finished all the plots. Looking towards the shore, our samples and coolers were being carried by the tide. It took all our effort to traverse back to the shore, and on the way retrieve the heavy, runaway samples.

 

Danielle is a rising senior at the College of William and Mary. As a ZENtern she will be working with the lab of Dr. Mary O’Connor at the University of British Columbia in Vancouver, as well as back at the Virginia Institute of Marine Science with Dr. Emmett Duffy’s Marine Biodiversity Lab during summer 2014. She has been volunteering in Dr. Duffy’s lab for the past two years and is from Massachusetts. Aside from her passion for science, Danielle is an avid dancer.

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